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KMID : 0900920020260010061
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Korean journal of Animal Reproduction
2002 Volume.26 No. 1 p.61 ~ p.68
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Effect of Aesculetin and O_2Concentrations on In Vitro Development of Preimplantation Embryo in Hanwoo (Korean Native Cattle)
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Jang H.-Y.
Park K.-E.
Kim C.-I.
Park C.-K.
Cheong H.-T.
Yang B.-K.
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Abstract
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The present study was examined effects of aesculetin and O_2concentrations on in vitro development of Hanwoo (Korean Native Cattle) embryos derived from in-vitro matured and fertilized (IVM-lVF) oocytes. The oocytes were cultured for the first 40~44 h after in vitro fertilization, then embryos of 2 to 8 cell stages were cultured under the different culture conditions for another 6 days. In experiment 1, the higher rates of morulae and blastocysts were produced in 5% O_2, than in 20% O_2(P＜0.05). There was significantly (P＜0.05) higher in embryos cultured with 1 mutextrm{g}/mell than with 0, 5 and 10 mutextrm{g}/mell of aesculetin. In experiment 2, the proportions of embryo developed with blastocysts and morulae plus blastocysts in 5% O_2, again was significantly (P＜0.05) higher in 20% O_2, during the culture with aesculetin and/or taurine. In the 5 and 20% O_2atmosphere, the inclusion of 1 mutextrm{g}/mell aesculetin or 2.5mM taurine increased significantly (P＜0.05) the percentages of blastocysts and morulae plus blastocysts. In experiment 3, in medium with aesculetin plus PDGF and taurine plus EGF than other treatment groups, significantly (P＜0.05) higher developmental rates were obtained. Number of blastomere in balstocyst stage were also higher in medium with that than without aesculetin. However, there were no significant differences in all culture conditions. In experiment 4, the proportions of embryo developed to the morulae and blastocyst stages were significantly (P＜0.05) higher rates in medium with natural and commercial aesculetin than in control medium. No significant differences, however, were observed in between natural (71%) and commercial (70.0%) aesculetin. Number of blastomere in blastocyst stage were also higher in medium with natural and commercial aesculetin than in control medium. However, there was no effect on the number of blastomeres by these treatment. These data indicate that preimplatation embryos are very sensitive to condition that can cause oxygen concentration and show that efficiency role of aesculetin for improving bovine embryo development in vitro.
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KEYWORD
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Aesculetin, Bovine, Embryonic Development, O_2, concentrations, Taurine
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